Composite
QS->RFP

Part:BBa_J85120:Design

Designed by: Cory Li   Group: Weiss Lab   (2010-06-01)


High Population->mRFP (pLac->LuxI; pLacIQ->luxR; pLux->mRFP;)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 863
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2502
    Illegal AgeI site found at 2614
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1864


Design Notes

Double terminator not used after LuxI because it is unnecessary and has the potential to make the plasmid more unstable.


Source

Built through assembly of BBa_K084014 and BBa_J10000.

References